Optimizing the regulation and control of sports injury and fatigue of winter olympic ice and snow athletes based on injury prevention / Otimização da regulação e controle de lesões esportivas e fadiga de atletas do gelo e da neve nos jogos olímpicos de inverno com base na prevenção de lesões / Optimización del control de lesiones deportivas y fatiga de los atletas de hielo y nieve en los juegos olímpicos de invierno basada en la prevención de lesiones deportivas

ABSTRACT This study reveals the characteristics and relationship of sports injury and fatigue of the Winter Olympics athletes, and monitors the athletes' psychological condition through case analysis of excellent athletes, and provides empirical evidence for athletes' psychological training and preparation for the physical and mental health services of Winter Olympics. Through questionnaires, literature review and other methods to study the injury situation of Winter Olympics ice and snow athletes, the results show that the proportion of chronic lumbar and knee joint injuries, and repeated muscle strains of Chinese Winter Olympics ice and snow athletes is large, and the cure rate is not high. The preparation activities are neither paid enough attention or sufficient, and the rationality of training plan arrangement is insufficient. The main cause of injury is insufficient understanding of the mechanism of injury and illness among athletes and coaches. Individual elite athletes need to improve their coping skills and pay attention to their psychological fatigue. In view of the sports' psychological characteristics and the problems faced by winter athletes, personalized psychological intervention programs should be formulated.

RESUMO O presente estudo revela as características e a relação de lesões desportivas e fadiga de atletas participantes dos Jogos Olímpicos de Inverno, e monitora as condições psicológicas dos atletas através da análise de casos de atletas de alto desempenho, além de apresentar provas empíricas para o treino e a preparação psicológico dos atletas para os serviços de saúde física e mental dos Jogos de Inverno. Através de questionários, revisão de literatura e outros métodos de estudo das lesões que acometem os atletas dos Jogos Olímpicos de Inverno, em esportes que envolvem gelo e neve, os resultados mostram que é grande a proporção de lesões lombares crônicas, lesões articulares do joelho e recorrência de distensões musculares, e a taxa de cura não é alta. As atividades de preparação não são suficientemente consideradas e a racionalidade do plano de treinos é insuficiente. A principal causa de lesão é a falta de compreensão dos mecanismos que ocasionam lesões e enfermidades entre atletas e treinadores. Atletas individuais de elite precisam melhorar suas habilidades de enfrentamento e prestar atenção à sua fadiga psicológica. Tendo em conta as características psicológicas desportivas e os problemas existentes entre os atletas dos Jogos de Inverno, programas de intervenção psicológica personalizados devem ser formulados.

RESUMEN A través del análisis de casos de atletas de élite, este trabajo revela las características y la relación entre las lesiones deportivas y la fatiga de los atletas en los Juegos Olímpicos de invierno, monitorea el estado psicológico de los atletas y proporciona evidencia empírica para el entrenamiento psicológico de los atletas y la preparación del Servicio de salud física y mental En los Juegos Olímpicos de invierno.Juegos Olímpicos.Los resultados mostraron que la proporción de lesiones crónicas de la cintura y la rodilla y la tensión muscular repetida de los atletas de hielo y nieve en los Juegos Olímpicos de invierno en China era mayor.La tasa de curación no es alta.No se presta suficiente atención a las actividades preparatorias y a la racionalidad del plan de capacitación.La razón principal de la lesión es que los atletas y entrenadores no entienden el mecanismo de la lesión y la enfermedad.Los atletas individuales deben mejorar su capacidad de enfrentamiento y prestar atención a la fatiga psicológica.De acuerdo con las características psicológicas de los atletas de invierno y los problemas existentes, se formula un plan de Intervención Psicológica individualizado.

MicroRNA-101 regulates T-cell acute lymphoblastic leukemia progression and chemotherapeutic sensitivity by targeting Notch1.

The present study aimed to investigate the role of microRNA (miR)-101 in acute lymphoblastic leukemia progression and chemoresistance. Furthermore, a novel target gene of miR-101 was identified. Here, we confirmed that miR-101 was significantly downregulated in the blood samples of patients with T-cell acute lymphoblastic leukemia (T-ALL) compared with the healthy controls, as determined by reverse transcription quantitative polymerase chain reaction (RTqPCR) analysis. The in vitro experiments demonstrated that miR-101 significantly repressed the proliferation and invasion, and induced potent apoptosis in Jurkat cells, as determined by CCK-8, flow cytometer and cell invasion assays. Luciferase assay confirmed that Notch1 was a target gene of miR-101, and western blotting showed that miR-101 suppressed the expression of Notch1 at the protein level. Moreover, functional restoration assays revealed that Notch1 mediates the effects of miR-101 on Jurkat cell proliferation, apoptosis and invasion. miR-101 enhanced the sensitivity of Jurkat cells to the chemotherapeutic agent adriamycin. Taken together, our results show for the first time that miR-101 acts as a tumor suppressor in T-cell acute lymphoblastic leukaemia and it could enhance chemotherapeutic sensitivity. Furthermore, Notch1 was identified to be a novel target of miR-101. This study indicates that miR-101 may represent a potential therapeutic target for T-cell acute lymphoblastic leukemia intervention.

PTTG regulates the metabolic switch of ovarian cancer cells via the c-myc pathway.

Human pituitary tumor-transforming gene (PTTG) is a proto-oncogene involved in the development, invasion, and metastasis of many types of cancer, including ovarian cancer. However, little is known about the role of PTTG in the metabolic shift of ovarian cancer cells. In our study, we show that PTTG expression was positively correlated with the differentiation degree of ovarian cancer tissue. In addition, PTTG suppression by specific shRNA could inhibit the proliferation of ovarian cancer cells A2780 and SKOV-3. Furthermore, aerobic glycolysis was suppressed and oxidative phosphorylation was increased in ovarian cancer cells after PTTG suppression. We further found that the expression of c-myc and several crucial enzymes involved in aerobic glycolysis (e.g., PKM2, LDHA, and glucose transporter 1 (GLUT-1)) were downregulated by PTTG knockwown. Overexpression of c-myc could prevent the metabolic shift induced by PTTG knockwown. Together, our findings suggest that the oncogene PTTG promotes the progression of ovarian cancer cells, and its loss resists tumor development, in part, by regulating cellular metabolic reprogramming that supports cell growth and proliferation via c-myc pathway.